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12.06.2018 - 02:02. It's no good to try to predict whether Moscow would ever have such an attack. Defending itself against this could cost the same as it would cost to.5.4 mm to the agar, and the plates were incubated at 37°C in 5% CO~2~for 48 hours. Authors' contributions ======================= Wei Guo performed the sequence comparison, genome analysis and drafted the manuscript. Jie-Ming Liu conceived of the study, participated in its design and coordination and helped to draft the manuscript. Chang-Qing Fu, Meng-Qiang Ye, Bing-Chun Zhou and Qing Li carried out the microbial cultivation and molecular analysis. Wei-Dong Zou carried out the whole-genome sequence assembly and participated in the genome analysis. Xing-Jian Liu and Hai-Peng Zhou participated in the design and coordination of the study. All authors read and approved the final manuscript. Acknowledgements ================ This work was supported by the "Special Fund for Agro-scientific Research in the Public Interest" (No.200803008), "National Science Foundation of China" (No. 30970154) and "863 Program" (No. 2010AA09Z412). We are grateful to Zhenchuan Liu, Chun-Guang Wu and Zong-Wei Tan for their help in this work. Figures and Tables ================== ![**Schematic diagram for the integration of the three comparative genomics methods: sequence alignment, phylogenetic analysis and gene neighborhood comparison**. Genomic regions were first aligned with the reference genome to detect the shared sequences, and a BLAST algorithm was used to detect the unaligned sequences. The shared sequences were further analyzed by a neighbor joining method, and the unaligned sequences were then aligned with each other by ClustalW to obtain phylogenetic information. The phylogenetic trees were visualized and analyzed using MEGA. The gene-neighborhood analysis was implemented by combining the BLAST program, the ClustalW program and the MEGA software.](1471-2180-8-104-1){#F1} ![**Virulence factor analysis of strain B5**. T3SS: T3 secretion system; T6SS: T6 secretion system; T8SS: T8 secretion system; LPS





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